Triplobiome™ Technology: set of bioactive compounds for safe skin

The modern consumer is increasingly seeking skincare solutions that are not only effective but also safe and derived from natural sources. Addressing this demand, Provital has pioneered Triplobiome™ Technology, a highly innovative biotech platform that harnesses the potent relationships between plants and their internal symbiotic microorganisms, the endophytes.

By leveraging the exchange of bioactivity between plants and these microorganisms in the natural world—Triplobiome™ brings a transformative approach to beauty by infusing beauty products with the adaptive and protective power of nature itself. Triplobiome™ Technology champions an era of eco-responsible beauty by developing fully traceable, sustainable ingredients. This technology represents a paradigm shift, integrating the synergistic power of the Animal and Plant kingdoms, along with their Microbiome, to create novel cosmetic ingredients. Examples of this groundbreaking technology in action include Pureblome™ and Shiloxome™, Provital’s first “ecosystem ingredients”.

Bioactive compounds: mechanisms of action and benefits for skin health

The efficacy of skincare products is largely attributed to their bioactive compounds, which exert beneficial effects through various mechanisms. These compounds, whether from plant extracts or biotechnological processes, can exhibit significant anti-inflammatory and antimicrobial properties.

Antioxidant properties and protection against environmental stressors

Many bioactive compounds act as potent antioxidants, scavenging free radicals and protecting the skin from damage caused by environmental stressors. Triplobiome™ Technology isolates endophytes that provide plants with resilience and protection against these stressors, transferring these benefits to the skin.

The first resulting ingredient of this endophyte isolation process is Shiloxome™,  derived from the endophytic yeast Kwoniella mangroviensis found in cork oak. After a metabolomic analysis of the ferment used to create this ingredient Provital identified bioactive compounds such as hydroxylated C18 fatty acids, phenyllactic acid, 5-methoxyindoleacetate, and succinic acid. These bioactive compounds possess interesting bioactive properties such as antimicrobial, antioxidant, and collagen degradation inhibition. Interestingly, some of them (e.g., phenyllactic acid and some indoleacetic acid derivatives) are well-established plant growth promoters (produced by yeast – endophytes) that stimulate both rapid and long-term responses in plants by regulation of various developmental and physiological processes (Maki et al., 2022; Limtong et al., 2014).  Therefore, Shiloxome™’s restructuring effect on the skin is the result of transferring a natural endophytic protective system to the skin.

On the other hand, the metabolomic analysis of Pureblome™ -a second endophyte-derived ingredient, isolated from the endophytic bacteria Bacillus velezensis found in the leaves of the hyssop – identified several bioactive compounds coming from both plant and bacterium. For instance, the peptides under 8 kDa identified, hold broad spectrum antioxidant properties. Surfactin, a lipopeptide also identified, shows significant antioxidant activity too, reducing lipid peroxidation and protecting against oxidative stress (Islam et al., 2022; MM Khan et al., 2020; Suchi et al., 2023).

Hydration and barrier function enhancement

Maintaining optimal skin hydration and a robust barrier is crucial. Certain bioactive compounds, including those found in plant extracts and produced by endophytes, can help retain water and support the skin’s lipid barrier.

A clear example is Shiloxome™’s effect in fortifying the skin’s microbiome barrier and reinforcing the integrity of the lipid barrier, ensuring improved moisture retention and proper epidermal function. Lipidomic analysis revealed that Shiloxome™ regulates hundreds of lipids, significantly increasing its chain lengths. This is particularly true for ceramides, which are strongly associated with a robust skin barrier function. An in vivoenrichment analysis showed that Shiloxome™ increases the total chain length of the lipidome.

In the case of Pureblome™, a specific bioactive lipopeptide (Fengycin) was identified in the ferment used for the creation of the ingredient. This compound is directly associated with anti-fungi and skin barrier strengthening.   (Vanittanakom et al., 1986).

Anti-inflammatory and skin-soothing effects

Inflammation is a key factor in various skin issues, and several bioactive compounds can help mitigate these issues through their anti-inflammatory properties.

As explained above, the metabolomic analysis of Shiloxome™ identified phenyllactic acid, which has antimicrobial and antioxidant properties. Shiloxome™, in vitro, showed a dose-dependent reduction of 5 inflammation mediators (CCL5, COX2, CSF2, GRO1 and TNFA).

In this regard, Pureblome™ was also proven to offer anti-inflammatory effects thanks, in part, to the small peptides (under 8 kDa) and the Dihydroxy-eudesmanolide, both identified through metabolomics, which hold anti-inflammatory properties (Padilla-González et al., 2016); as well as the lipopeptide surfactin, that reduces inflammation in sensitive or damaged skin (Zouari et al., 2016).  Besides looking at the bioactive compounds found in its ferment, Pureblome™ achieves “the right balance” with its proven anti-inflammaging action. Invitro, for instance,it reduced the secretion of pro-inflammatory cytokines IL-6 and IL-8 in sebocytes treated with linoleic acid.

Advances in bioactive compounds for skin aging

The quest for youthful skin has driven significant research into bioactive compounds that can address the signs of aging.

Stimulating collagen and elastin production

Bioactive factors can stimulate fibroblasts to produce collagen and elastin, the skin’s structural proteins.

In the case of Shiloxome™, succinic acid was identified in the previously-mentioned metabolomic analysis. This bioactive compound has properties related to collagen degradation inhibition (Maki et al., 2022). In turn, the treatment on human dermal fibroblasts showed an enrichment of pathways related to the extracellular matrix remodeling and an improvement in dermal collagen and elastin content. A global transcriptome enrichment analysis indicated that Shiloxome™ triggers pathways related to extracellular matrix structure and organization.

Pureblome™ has demonstrated the ability to stimulate collagen and elastin production by revitalizing fibroblasts and decreasing glycation in vitro. Interestingly, the lipopeptide surfactin, identified in its ferment is also known to stimulate collagen and inhibit matrix metallopeptidases (Bueno-Mancebo et al., 2024).

Reducing wrinkles and fine lines

By promoting collagen synthesis and inhibiting enzymes that degrade it, certain bioactive compounds can help reduce the appearance of wrinkles and fine lines.

Shiloxome™ also contributes to a smoother skin microstructure, with an isotropic orientation of the skin surface being statistically significant at day 56 of in vivo testing.

In vivo studies have shown that a 2% Pureblome™ formulation significantly reduced wrinkles after only 14 days, as observed through profilometry analysis. In this case, the lipopeptide mentioned above is also linked to wrinkle reduction (Bueno-Mancebo et al., 2024).

Combating oxidative damage and glycation

Protecting the skin from oxidative damage and glycation (sugar-induced protein damage) is vital for preventing premature aging.

When it comes to bioactive compounds associated, we could mention Broussonetinine A, a plant metabolite identified through metabolomics in the ferment used to create  Pureblome™ It reduces glucose-related damage, hence preventing glycation end products (AGEs) (Zhong et al., 2025). Consequently, Pureblome™  showed, in vitro, an important anti-glycation capacity by significantly reducing Advanced Glycation End products (AGEs).

Bioactive compounds in dermatological research

Bioactive compounds are increasingly recognized for their potential in treating various skin conditions.

Potential in treating acne and other skin conditions

Many bioactive compounds exhibit antimicrobial and anti-inflammatory activities, making them promising for acne-prone skin.

Pureblome™ targets the root causes of adult acne by regulating sebocyte activity, balancing sebum production, and reducing acne-related factors. In this direction, this ingredient is highly associated to anti-acne compounds from both bacterial and plant origin (Bagatin et al., 2019):

– Its peptides under 8 kDa target opportunistic bacteria in acne pathogenesis and its associated skin inflammation (Alessandrini et al., 2023).

– Surfactin disrupts C. acnes cell walls, reduces inflammation, and alleviates redness and swelling in acne-prone skin (Shan et al., 2022).

– Iturin has broad-spectrum activity against bacteria and fungi and prevents skin infections.

– Dihydroxy-eudesmanolide combats microbial infections (Yaraguppi et al., 2023).

There is no wonder why Pureblome™’s in vitro and in vivo studies demonstrated a significant decrease in  skin lipids and a statistically significant reduction of protoporphyrins-IX (a pathogenic microbial marker associated with acne) (Saint-Leger et al., 1986).

Supporting skin regeneration and wound healing

Certain bioactive compounds, play a crucial role in skin regeneration and wound healing.

Pureblome™ has shown to increase fibroblast migration by +77 in in vitro studies, and to decrease skin turnover time (SCTT) by 1.7 days vs placebo, in vivo . This widely proven skin regenerative power could be partially associated its metabolomically-identified bioactive compounds like Surfactin, which accelerates wound healing (Zouari et al., 2016) and Iturin, which promotes faster dermal repair (Yaraguppi et al., 2023).

Natural sources of bioactive compounds: plant-based solutions for skin wellness

The plant kingdom is an extensive source of bioactive compounds beneficial for skin wellness. Triplobiome™ Technology specifically utilizes this natural wealth by focusing on the symbiotic relationship between plants and their endophytes.

For Pureblome™, the endophytic bacteria Bacillus velezensis is isolated from the leaves of agroecological hyssop (Hissopus officinalis), a plant valued for its antimicrobial properties since ancient times. The creation process involves isolating this highly bioactive endophyte and bio-optimizing it through controlled bio-fermentation.

Shiloxome™ is derived from the endophytic yeast Kwoniella mangroviensis found inside the bark of the cork oak (Quercus suber), a tree known for its longevity and resilience. Provital isolates relevant endophytic strains and performs bioactivity screenings to select candidates like the one used for Shiloxome™. Metabolomic analysis of the K. mangroviensis ferment revealed bioactive compounds such as the ones mentioned in this article, demonstrating the synergistic efficacy of the active metabolites obtained through the symbiotic relationship.

Triplobiome ™ Technology: a revolution in skin protection and regeneration

Triplobiome™ Technology represents a true revolution by strategically leveraging interkingdom symbiosis to achieve unprecedented synergistic effects in cosmetic ingredient development.

The role of Triplobiome ™ in enhancing the effectiveness of bioactive compounds

By mimicking the dynamic exchange between plants and their endophytes, Triplobiome™ taps into nature’s wisdom, transferring adaptive and protective benefits to the skin. This innovative platform allows for the sustainable production of diverse plant metabolites, offering multifaceted benefits for skincare. The technology aims to activate the skin’s inherent intelligence by transferring nature’s ancient wisdom through unique biological interactions.

Synergy of plant, microorganisms, and algae bioactives

Provital’s Triplobiome™ Technology primarily focuses on the synergistic relationship between plants and their internal microbiome, the endophytes (bacteria and fungi like yeast). The platform integrates the power of the Animal and Plant kingdoms, along with their microbiome. While current flagship ingredients focus on plant-endophyte symbiosis, the concept of “ecosystem ingredients” and “interkingdom symbiosis” suggests a broader potential for incorporating other natural symbiotic relationships in future developments, possibly including algae-derived bioactives given algae’s known skin health benefits.

Bioactive compounds from nature for skin wellness

Triplobiome™ develops ecosystem ingredients that are 100% traceable, eco-responsible, and biotechnologically obtained, delivering nature’s balance, resilience, and regenerative power directly to the skin. This approach aligns with the increasing consumer demand for nature-derived and sustainable beauty solutions.

Triplobiome ™ and the microbiome: the key to optimizing skin protection

Triplobiome™ Technology goes beyond traditional approaches by introducing postbiotic ingredients derived from the plant’s internal microbiota.

Shiloxome™, as a prime example, provides multi-ome skin protection by shielding the skin from environmental pollutants and crucially, by protecting the skin microbiome barrier and maintaining its natural diversity. In vivo microbial diversity analysis showed that Shiloxome™ did not alter the skin microbiota diversity (measured by Shannon and Simpson indexes) over 56 days, indicating its ability to maintain the skin’s natural microbiome balance. A healthy, balanced skin microbiome acts as a key ally against the exposome, helping to maintain overall skin health. By the robust reinforcement of the lipid barrier, as evidenced by comprehensive lipidomic studies showing an increase in the total chain length of the lipidome and regulation of long-chained ceramides, Shiloxome™ ensures improved moisture retention and enhanced “brick & mortar” epidermal function.

Pureblome™ also demonstrates its impact on the skin’s microbial balance by showing a clinically relevant decrease in protoporphyrins-IX, a pathogenic microbial marker associated with acne.

For further information or insights on bioactive compounds, please do not hesitate to contact our team of experts, who are available to provide guidance and support in selecting the most suitable solutions for your requirements.